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eia optical density (od 450)  (IDEXX)


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    IDEXX eia optical density (od 450)
    The fibril stability <t>of</t> <t>PrP</t> Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by <t>EIA,</t> expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates
    Eia Optical Density (Od 450), supplied by IDEXX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eia optical density (od 450)/product/IDEXX
    Average 90 stars, based on 1 article reviews
    eia optical density (od 450) - by Bioz Stars, 2026-05
    90/100 stars

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    1) Product Images from "Pathologic and biochemical characterization of PrP Sc from elk with PRNP polymorphisms at codon 132 after experimental infection with the chronic wasting disease agent"

    Article Title: Pathologic and biochemical characterization of PrP Sc from elk with PRNP polymorphisms at codon 132 after experimental infection with the chronic wasting disease agent

    Journal: BMC Veterinary Research

    doi: 10.1186/s12917-018-1400-9

    The fibril stability of PrP Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by EIA, expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates
    Figure Legend Snippet: The fibril stability of PrP Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by EIA, expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates

    Techniques Used: Infection, Incubation, Concentration Assay, Comparison

    The relative amount of PrP Sc in the brainstem (obex) is strongly associated with incubation period. The amount of PrP Sc in the brain was calculated using optical density readings from an antigen-capture enzyme immunoassay (EIA). The relative amount of PrP Sc in the brain of the elk with the lowest EIA result in the linear range was designated a baseline value of 1.0. Results for other elk are expressed as a ratio relative to the baseline elk. Frozen obex was not available for elk #2 (MM132) so this animal is not included in the figure
    Figure Legend Snippet: The relative amount of PrP Sc in the brainstem (obex) is strongly associated with incubation period. The amount of PrP Sc in the brain was calculated using optical density readings from an antigen-capture enzyme immunoassay (EIA). The relative amount of PrP Sc in the brain of the elk with the lowest EIA result in the linear range was designated a baseline value of 1.0. Results for other elk are expressed as a ratio relative to the baseline elk. Frozen obex was not available for elk #2 (MM132) so this animal is not included in the figure

    Techniques Used: Incubation, Enzyme-linked Immunosorbent Assay



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    eia optical density (od 450)  (IDEXX)
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    IDEXX eia optical density (od 450)
    The fibril stability <t>of</t> <t>PrP</t> Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by <t>EIA,</t> expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates
    Eia Optical Density (Od 450), supplied by IDEXX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eia optical density (od 450)/product/IDEXX
    Average 90 stars, based on 1 article reviews
    eia optical density (od 450) - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

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    The fibril stability of PrP Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by EIA, expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates

    Journal: BMC Veterinary Research

    Article Title: Pathologic and biochemical characterization of PrP Sc from elk with PRNP polymorphisms at codon 132 after experimental infection with the chronic wasting disease agent

    doi: 10.1186/s12917-018-1400-9

    Figure Lengend Snippet: The fibril stability of PrP Sc from MM132 and LM132 elk was lower than the fibril stability of PrP Sc from LL132 elk. Homogenates of infected elk brain were incubated in GdnHCl at the indicated concentration as described in Methods, with remaining PrP Sc , as detected by EIA, expressed as a fraction of the signal after treatment with 0.25 M GdnHCl. a Comparison of individual animals of MM132, LM132 and LL132 genotype elk, as indicated. Data were averaged across 4–6 technical replicates to generate each curve. b Average curves for LL132 elk (closed symbols) as compared to MM132 and LM132 elk (open symbols) from ( a ); error bars depict +/− the standard error of the mean (SEM) of the biological replicates

    Article Snippet: The relative amount of PrP Sc remaining was assessed by the EIA optical density (OD 450 ) after dilution of treated brain homogenates to a final [GdnHCl] of 0.25 M and application to the IDEXX plate.

    Techniques: Infection, Incubation, Concentration Assay, Comparison

    The relative amount of PrP Sc in the brainstem (obex) is strongly associated with incubation period. The amount of PrP Sc in the brain was calculated using optical density readings from an antigen-capture enzyme immunoassay (EIA). The relative amount of PrP Sc in the brain of the elk with the lowest EIA result in the linear range was designated a baseline value of 1.0. Results for other elk are expressed as a ratio relative to the baseline elk. Frozen obex was not available for elk #2 (MM132) so this animal is not included in the figure

    Journal: BMC Veterinary Research

    Article Title: Pathologic and biochemical characterization of PrP Sc from elk with PRNP polymorphisms at codon 132 after experimental infection with the chronic wasting disease agent

    doi: 10.1186/s12917-018-1400-9

    Figure Lengend Snippet: The relative amount of PrP Sc in the brainstem (obex) is strongly associated with incubation period. The amount of PrP Sc in the brain was calculated using optical density readings from an antigen-capture enzyme immunoassay (EIA). The relative amount of PrP Sc in the brain of the elk with the lowest EIA result in the linear range was designated a baseline value of 1.0. Results for other elk are expressed as a ratio relative to the baseline elk. Frozen obex was not available for elk #2 (MM132) so this animal is not included in the figure

    Article Snippet: The relative amount of PrP Sc remaining was assessed by the EIA optical density (OD 450 ) after dilution of treated brain homogenates to a final [GdnHCl] of 0.25 M and application to the IDEXX plate.

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay